Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| T4 Holin |
BBa_K112000 |
membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane |
| Cathelicidin LL-37 |
|
antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs |
Topology
Parts
| Name |
Number |
Comment |
| pCons |
BBa_J23100 |
constitutive promotor |
| CymR |
|
cumate-responsive regulator CymR - can bind to T5 cumate operator |
| RBS |
|
|
| Ter |
|
|
| T5 cumate operator |
|
cumate responsive promotor inhibited by CymR |
| T4 Holin |
BBa_K112000 |
membrane protein from bacteriophage T4 - depolarizes and permeabilizes the membrane |
| Cathelicidin LL-37 |
|
antimicrobial peptide which can associate with the bacterial membrane and form pores - cell lysis occurs |